产品说明
细胞系来源
Trout Rainbow gonad tissue
细胞系描述
From pooled male/female gonad tissue of yearling rainbow trout (Oncorhynchus mykiss). First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24℃. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE).
应用
Virus studies: Inf. pacr. necrotic virus, Egtved virus, arboviruses (EEE, VEE)
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Passage using 0.25% trypsin below 24℃. Cells may be seeded as few as 3x10,000 cells/ml 5% CO2; 24℃. Fish cell lines detach easily during transit if the culture is very youn
其他说明
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产品性质
| 生物来源 | fish ovary |
| 生长模式 | Adherent |
| 核型 | 2n = 60 |
| 形态学 | Fibroblast |
| 产品 | Not specified |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | −196℃ |
