H-4-II-E

产品说明

细胞系来源

Rat hepatoma Reuber H35

细胞系描述

Exhibits high arylhydrocarbon hydroxylase inducibility and is used to detect picogram levels of planar polychlorinated organic compounds in food extracts and other environmental samples.

应用

Hormone induced enzymatic studies

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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产品性质