Do-11-10

产品说明

细胞系来源

Mouse BALB/c (H-2d) T cells x Mouse AKR Thyoma, BW5147

细胞系描述

Do-11-11 was produced by fusion of the antigen-specific I region-restricted (H-2d) BALB/c T-cell blasts with the AKR thyoma BW5147. This murine helper T cell hybridoma is specific for chicken ovalbumin (cOva) restricted to I-Ad and cross reacts with cOva I-antibody. The hybrid can be stimulated to secrete Interleukin 2 (IL-2) or to act as an antigen-specific helper T-cell. Expression of IL-2 can be lost by long-term culture of growth at high densities.

培养基

DMEM or RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

传代培养常规

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37 ℃. Cells should not be grown at high density; avoid long-term culture as this might lead to change of cell receptors.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

产品性质