产品说明
细胞系来源
Mouse (CxDBA)F1 melanoma
细胞系描述
Established from a Cloudman S91 melanoma in a male mouse; the cells produce melanin.
应用
Cloning studies
培养基
Ham′s F10 + 2mM Glutamine + 10% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or 15% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 2-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃.
其他说明
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产品性质
生物来源 | mouse skin |
生长模式 | Adherent |
核型 | Hypertetraploid |
形态学 | Epithelial |
产品 | Melanin |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | −196℃ |