产品说明
细胞系来源
Mouse LAF1 pituitary gland tumour
细胞系描述
Derived after alternate passage of LAF1 mouse pituitary tumour cells as tumours in animals and in cell culture. Cells produce adrenocorticotropic hormone (ACTH) at approximately the rate of 1000mU/mg of cell protein per week.
应用
Hormone studies
培养基
Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS). Cells grow in large aggregates.
传代培养常规
Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. A subcultivation ratio of 1:2 is recommended. Cells grow in large viable clusters making counting difficult. A seeding densi
其他说明
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产品性质
| 生物来源 | mouse brain |
| 生长模式 | Aggregates in suspension |
| 核型 | Diploid |
| 形态学 | Small round cells growing as large aggregates |
| 产品 | Adrenocorticotropic hormone (ACTH) |
| 受体 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| 相关疾病 | cancer |
| 运输 | dry ice |
| 储存温度 | −196℃ |
