产品说明
一般描述
Betaine also called trimethylglycine or N,N,N triethylammonium acetate, is an analog of glycine with three methyl groups. It is highly compatible with polymerase chain reaction (PCR) buffer mixture. Betaine is a PCR enhancing reagent that is widely used for improving the yield and specificity of PCR products, especially during the PCR amplification of targets rich in GC content or those that form secondary structures resulting in poor yield. Betaine facilitates DNA strand separation and manages the DNA melting temperature (Tm) difference between the GC and AT pairs in DNA. It stabilizes the ds DNA by equalizing the contribution of GC- and AT-base pairs. Betaine has been broadly used to optimize multiplex and ‘long and accurate′ polymerase chain reaction (LA-PCR). The addition of 1.0-1.7 M aqueous betaine to a PCR mixture has been reported to reduce the base pair composition dependence on DNA strand melting.
应用
甜菜碱溶液已被用作以下组分
- 环介导等温扩增(LAMP)反应
- 用于基因组DNA扩增的聚合酶链式反应
- 用于端粒长度分析的定量聚合酶链式反应(qPCR)
- PCR扩增基因组DNA之中的CGG重复片片段。
包装
每管1.5mL
基本信息
| 线性分子式 | (CH3)3N+CH2COO− |
| 分子量 | 117.15 |
| Beilstein | 3537113 |
| MDL编号 | MFCD00012123 |
| PubChem化学物质编号 | 24891511 |
产品性质
| 质量水平 | 300 |
| 等级 | PCR Reagent |
| 形式 | liquid |
| 包装 | vial of 1.5 mL |
| 浓度 | 5 M |
| technique(s) | PCR: suitable |
| 颜色 | colorless |
| application(s) | agriculture |
| 异质活性 | DNase, RNase, none detected |
| 储存温度 | 2-8℃ |
| SMILES string | C[N+](C)(C)CC([O-])=O |
| InChI | 1S/C5H11NO2/c1-6(2,3)4-5(7)8/h4H2,1-3H3 |
| InChI key | KWIUHFFTVRNATP-UHFFFAOYSA-N |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
