Pyrophosphate reagent

产品说明

应用

The product has been used for the measurement of pyrophosphate content of plant tissues. It has also been used as a rapid, routine check of pyrophosphate content by monitoring NADH oxidation in Solanum tuberosum tuber extracts.

生化/生理作用

The pyrophosphate detection kit couples the production of pyrophosphate to the consumption of NADH.

包装

A technical bulletin is included.

组分

ComponentConcentration
Imidazole × HCl, pH 7.4 45 mM
Citrate 5 mM
EDTA 0.10 mM
Mg²⁺, Mn²⁺, Co²⁺ 2 mM, 0.2 mM, 0.02 mM
β-NADH 0.8 mM
F-6-P 12 mM
Bovine Serum Albumin 5 mg/mL
Sugar Stabilizer 5 mg/mL
PPi-PFK 0.5 units/mL
Aldolase 7.5 units/mL
GDH 5 units/mL
TPI 50 units/mL

质量

A coupled enzyme system containing fructose-6-phosphate kinase (pyrophosphate-dependent), aldolase, triosephosphate isomerase, and α-glycerophosphate dehydrogenase.

原理

Two moles of NADH are oxidized to NAD⁺ per mole of pyrophosphate consumed. The reaction is monitored spectrophotometrically at 340 nm.
The reagent can be used to detect 10-100 nmoles of pyrophosphate.

制备说明

Dissolve the contents of one vial of Pyrophosphate Reagent in 4.0 mL of water.

基本信息

产品性质

安全信息