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L-Glutamate Oxidase from Streptomyces sp.

L -谷氨酸氧化酶 来源于链霉菌

品牌
Sigma-Aldrich
CAS
39346-34-4
货号
G1924
规格纯度
recombinant, expressed in E. coli
参考价格
1535.43 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

This product has a 6xHistidine tag with a thrombin cleavage site.
L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.

应用

L-Glutamate Oxidase from Streptomyces sp may be used:

  • as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development
  • as a component of pencil graphite electrode for biosensing L-glutamate

生化/生理作用

L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.

单位定义

One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.

基本信息

MDL编号MFCD00131459
NACRESNA.54

产品性质

质量水平200
重组expressed in E. coli
形式solid
specific activity≥5 units/mg protein
分子量 120 kDa
储存温度−20℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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