产品说明
一般描述
Lipases hydrolyze ester bonds of triacylglycerols and in some conditions, catalyze the synthesis of ester bonds by transesterification. Lipase from Rhizopus niveus exists as a single polypeptide chain with a molecular mass of about 30-34kDa.
应用
Lipase from Rhizopus niveus has been used to determine the positional distribution of fatty acids within triacylglycerol by hydrolysis of the acyl ester linkage at C-1 and C-3. It has also been used to induce intragastric lipolysis and study its effects on felodipine (a medication) release from a hydrophilic, extended release tablet.
生化/生理作用
Lipase from Rhizopus niveus is specifically used to produce cocoa butter substitutes.
可水解甘油三酯、甘油二酯、甘油单酯(速率依次递减)。
单位定义
1U 相当于在 pH 7.7 和 40℃ 下每分钟从甘油三酯转化出 1μmol 脂肪酸所需的酶量(以橄榄油为底物)；如上所述的 300U 相当于在 pH 8.0 和 40℃ 下，以 Fluka® 编号 62314 的甘油三油酸酯作为底物时的 ~1U。
其他说明
说明：以三乙酰甘油酯作为底物时，pH 为 7.4。孵化期：60 分钟。
底物特异性：优先水解 1- 和 3-位(长链脂肪酸的中部)；最佳 pH 5.0-7.0；最适温度 30-45℃；酶法选择性去除保护官能团(C 端 COOH 官能团)
基本信息
| 酶学委员会(EC)编号 | 3.1.1.3 ( BRENDA | IUBMB ) |
| EC 号 | 232-619-9 |
| MDL编号 | MFCD00131509 |
| NACRES | NA.26 |
产品性质
| 质量水平 | 100 |
| 生物来源 | fungus (Rhizopus niveus) |
| 形式 | powder (fine) |
| specific activity | ≥1.5 U/mg |
| 分子量 | Mr ~83000 |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, type N95 (US) |
