产品说明
一般描述
ExtrAvidin is a chemically modified protein purified from egg whites.
The extravidin-R-phycoerythrin protein can be conjugated with fluorescein-labelled arylacetamide, a κ opioid ligand and biotin-conjugated anti-fluorescein IgG to identify the subpopulations of thymocytes that express κ opioid receptor. This product has shown specificity for rabbit anti-avidin in immunoelectrophoresis analysis.
ExtrAvidin, a uniquely modified avidin reagent, combines the high specific activity and sensitivity of avidin with the low background staining of streptavidin. Avidin consists of four identical subunits, each containing a single biotin binding site.
免疫原
Thymocytes present in C57BL mice.
应用
ExtrAvidin®-R-Phycoerythrin has been used
- In flow cytometric analysis to confirm membrane expression of ncamp-1 (cationic oligodeoxynucleotide binding protein).
- In indirect immunofluorescence.
- In Western blotting.
The ExtrAvidin-R-Phycoerythrin (PE) conjugate may be used in conjunction with biotinylated reagents in the avidin/biotin labeling system in flow cytometry.
包装
1 mL in glass bottle
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide
法律信息
ExtrAvidin is a registered trademark of sigma-aldrich Co. LLC
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| MDL编号 | MFCD00163600 |
| NACRES | NA.46 |
产品性质
| 质量水平 | 200 |
| 偶联物 | phycoerythrin (R-PE) conjugate |
| 形式 | buffered aqueous solution |
| 储存条件 | protect from light |
| 标记范围 | 1-4 mol PE per mol ExtrAvidin® |
| technique(s) | flow cytometry: suitable using human peripheral blood lymphocytes with biotinylated antibody immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5 μg/mL using human tissues |
| 储存温度 | 2-8℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
