产品说明
一般描述
Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
应用
Lambda DNA Mixed Digest has been used for DNA electrophoresis.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.
组分
10mM Tris-HCl 中的溶液，pH 8.0，1mM EDTA
制备说明
Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.
其他说明
For optimal resolution, the recommended agarose gel concentration is 0.4%.
基本信息
| NACRES | NA.25 |
产品性质
| 质量水平 | 200 100 |
| 形式 | buffered aqueous solution |
| 用途 | 100 uses (0.1 mg size) 20 uses (20 μg size) |
| 适用性 | suitable for electrophoresis (DNA) |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
