产品说明
一般描述
codA基因编码胆碱氧化酶。
应用
西格玛公司生产的胆碱氧化酶已用于酶法测定牛奶中的胆碱，该方法采用了流动注射分析(FIA)系统和电位检测。†
生化/生理作用
Choline oxidase from Arthrobacter sp. is a flavoprotein, which is a member of the GMC-oxidoreductase family. The enzyme catalyzes the four-electron-oxidation of choline to glycine betaine via the intermediate betaine aldehyde, in two sequential FAD-dependent reaction steps. The enzyme is useful for enzymatic determination of phosphatidylcholine by coupling with phospholipase D or determination of sphingomyelin by coupling with sphingomyelinase and for cholinesterase activity assays.
Choline oxidase, isolated from the soil bacterium Arthrobacter globiformis helps to transform choline to glycinebetaine (N-trimethylglycine).
单位定义
在37 ℃条件下，当pH为8.0时，一个单位每分钟将形成1 μ摩尔的H2O2，其中1 μ摩尔胆碱氧化成甜菜醛。注：在胆碱氧化酶将胆碱转化为甜菜碱的过程中，为每μ摩尔的胆碱生成2 μ摩尔的H2O2。
制备说明
建议在补充10mM乙二胺四乙酸的pH 7.5磷酸盐缓冲液中重建胆碱氧化酶。重组材料可在2-8℃条件下储存至少2周。
基本信息
| 酶学委员会(EC)编号 | 1.1.3.17 ( BRENDA | IUBMB ) |
产品性质
| 质量水平 | 200 |
| 重组 | expressed in E. coli |
| specific activity | ≥12 units/mg protein |
| 储存温度 | −20℃ |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H334 - H412 |
| 预防措施声明 | P261 - P273 - P284 - P304 + P340 + P312 - P501 |
| 危险分类 | Aquatic Chronic 3 - Resp. Sens. 1 |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
