产品说明
细胞系来源
Mouse BALB/c testis sertoli cell
细胞系描述
From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.
应用
Hormone studies
培养基
Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37℃. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
产品性质
| 生物来源 | mouse testis |
| 生长模式 | Adherent |
| 核型 | Not specified |
| 形态学 | Not specified |
| 产品 | Transferrin, H-Y Ag, novel retinol-binding protein |
| 受体 | Androgen, oestrogen and progesterone receptors |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | −196℃ |
