产品说明
细胞系描述
Primary mouse embryonic fibroblasts (MEFs) were isolated from the embryos of a pregnant female Ulk1-/- Ulk2-/+ mouse at day 13.5 post coitus. The embryos were genotyped to identify those that were Ulk1 -/- Ulk2 -/- and the MEFs that were isolated and cultured were immortalised with SV40 large T-antigen.
培养基
DMEM + 10% FCS +2mM Glutamine
传代培养常规
Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 104 per cm2. Cultures must be incubated in a humidified 5% CO2/95% air incubator at 37oC.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
免责声明
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
产品性质
| 生物来源 | mouse embryo |
| 描述 | Mouse embryonic fibroblast, autophagy, Ulk1 -/- Ulk2 -/-, double knockout, SV40-immortalised |
| 生长模式 | Adherent |
| 形态学 | Fibroblast |
| technique(s) | cell culture | mammalian: suitable |
| 运输 | dry ice |
| 储存温度 | −196℃ |
