产品说明
应用
The enzyme from Sigma has been used to assess the effect of limited proteolysis with α-chymotrypsin on the sperm penetration.
α-Chymotrypsin from bovine has been used in a study to inform proteasome inhibition in order to advance anticancer research. α-Chymotrypsin from bovine has also been used in a study that functionalized surface anchored poly(methylhydrosiloxane) thin films on oxidized silicon wafers.
α-Chymotrypsin from bovine pancreas has been used:
- as a supplement for the collection of semen into Tris diluent
- as one of the proteases in the analysis of major histocompatibility complex (MHC) class II protease sensitivity assay
- as a component in YEPD broth for biofilm dispersion assay
- in the preparation of chitinase–chymotrypsin–DMSO buffer (CCD buffer) for enzymatic digestion of larvae
生化/生理作用
α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu2+ and Hg2+.
一种丝氨酸蛋白酶，能将与芳香烃或其他大型疏水性侧链(Tyr、Trp、Phe、Met、Leu)结合的肽从键的羧基端水解。
单位定义
在 pH 7.8 和 25℃ 下，一单位本品每分钟可水解 1.0μmol BTEE。
分析说明
由 A
基本信息
| 酶学委员会(EC)编号 | 3.4.21.1 ( BRENDA | IUBMB ) |
| EC 号 | 232-671-2 |
| MDL编号 | MFCD00130481 |
| NACRES | NA.54 |
产品性质
| 质量水平 | 200 |
| 描述 | aseptically filled |
| 类型 | Type IV-S |
| 形式 | solid |
| specific activity | ≥40 units/mg protein |
| 分子量 | 25 kDa |
| 组成 | protein, ≥85% UV |
| 包装 | vial of 5 mg |
| UniProt登记号 | P00767 |
| 储存温度 | −20℃ |
| Gene Information | cow ... CTRB1(618826) |
安全信息
| 象形图 |    |
| 警示用语： | Danger |
| 危险声明 | H302 - H315 - H319 - H334 - H335 - H400 |
| 预防措施声明 | P261 - P273 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
| 危险分类 | Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3 |
| 靶器官 | Respiratory system |
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 个人防护装备 | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
