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Phosphatase, Alkaline from calf intestinal mucosa

磷酸酶(碱性) 来源于小牛肠粘膜

品牌
Sigma-Aldrich
CAS
9001-78-9
货号
79390
规格纯度
suitable for enzyme immunoassay, solution (clear, colorless), ~2500 U/mg protein (~10 mg/ml)
参考价格
3611.69 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

应用

Alkaline phosphatase is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

生化/生理作用

Alkaline phosphatase is a hydrolytic enzyme which dephosphorylates a variety of molecules under basic conditions.

单位定义

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 4-nitrophenyl phosphate per minute at pH 9.8 and 37 ℃

重悬

The solution can be used without dialysis (for enzyme immuno-assay).

其他说明

Sales restrictions may apply
Used in highly sensitive (pg/m) enzyme immunoassays with LCEC detection of phenol; Comparison of three methods for conjugating alkaline phosphatase to human placental lactogen; Preparation of enzyme-antibody conjugates; Liposome immunoassay with antigen-coupled liposomes containing alkaline phosphatase

基本信息

酶学委员会(EC)编号3.1.3.1 ( BRENDA | IUBMB )
EC 号232-631-4
MDL编号MFCD00131849
eCl@ss42010105
NACRESNA.54

产品性质

形式solution (clear, colorless)
specific activity~2500 U/mg protein (~10 mg/ml)
分子量Mr ~140000
technique(s)enzyme immunoassay: suitable
杂质0.1 mM ZnCl2
1 mM MgCl2
3 M NaCl
30 mM triethanolamine
pH值(酸碱度)~7.6
储存温度2-8℃

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, type N95 (US)

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