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Transcreener® ADP2 TR-FRET Red Assay

品牌
Sigma-Aldrich
货号
3011
包装型号
规格纯度
参考价格
13214.2 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The Transcreener® ADP2 TR-FRET Red Assay is a competitive immunoassay for ADP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. Because it is highly selective for ADP, the assay can be used with any enzyme that converts ATP to ADP, regardless of what other substrates are used. Examples of enzymes include protein, lipid, and carbohydrate kinases, ATPases, DNA helicases, carboxylases, and glutamine synthetase.
The Transcreener® assay is designed specifically for high-throughput screening (HTS), with a single addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families.

The Transcreener® ADP2 TR-FRET Red Assay provides the following benefits:

  • Accommodates ATP concentrations ranging from 0.1 μM to 1,000 μM. • Excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10% or less).
  • Overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by using a single set of assay reagents that detect an invariant product.
  • Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds.
  • Far-red tracer further minimizes interference from fluorescent compounds and light scattering.

View full Transcreener® product list

数量

3011-1K = 1,000 assay, 384-well
3011-10K = 10,000 assay, 384-well

外形

Kit with buffered aqueous solutions

法律信息

Transcreener is a registered trademark of BellBrook Labs

基本信息

NACRESNA.84

产品性质

运输dry ice
储存温度−20℃

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