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Ribonuclease A from bovine pancreas

核糖核酸酶A 来源于牛胰腺

品牌
Sigma-Aldrich
CAS
9001-99-4
货号
R5250
规格纯度
Type X-A, ≥90% (SDS-PAGE), ≥70 Kunitz units/mg protein
参考价格
3667.63 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

RNase A(核糖核酸酶A)是一种内切核糖核酸酶,在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端(例如,pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG)。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同,并非糖蛋白。核糖核酸酶不会水解DNA,因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外,RNase也被DNA竞争性抑制。
Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can hydrolyze RNA from protein samples. Pancreatic RNase A specifically cleaves at the 3′-side of pyrimidine (uracil or cytosine) phosphate bonds. The ribonuclease (RNase) gene is mapped to human chromosome 14.

应用

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. Ribonuclease A is used for RNase protection assays, to remove unspecifically bound RNA, analysis of RNA sequences, to hydrolyze RNA contained in protein samples, and the purification of DNA. Ribonuclease A from bovine pancreas has been used in a study to assess the mechanism of heavy metal ions on RNase activity. Ribonuclease A from bovine pancreas has also been used in a study to investigate the performance of oligomeric poly(diallyldimethylammonium chloride) as displacer for cation-exchange displacement chromatography of proteins.

  • RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
  • RNase A还用于RNA序列分析和保护测定。
  • RNase A已用作计算辅助药物设计的工具。
  • RNase A为RNA序列分析提供支持。
  • RNase A水解蛋白质样品中的RNA。
  • RNase A为DNA纯化提供支持。

Ribonuclease A from bovine pancreas has been used to treat human glioblastoma U251 cell line prior to harvesting, for flow cytometry analysis, T suppressor cells and embryonic stem cell chromatin.

生化/生理作用

The structure of Ribonuclease A (RNase A) comprises of four disulfide and catalytic triad of two histidines crucial for its functionality. RNase A displays three-dimensional domain swapping of the α-helical N-terminal and the C-terminal β-strand. It can exist as dimer or oligomers. The level of RNase is elevated in cancers and infectious diseases. RNase A family of enzymes serve as potential drug targets. The homolog and variants of RNase A are potential chemotherapeutic agents.

特点和优势

我们高度稳定的核糖核酸酶A——RNase A,适合于RNA去除、RNA测序和DNA纯化。

外形

0.2M 磷酸钠缓冲溶液,pH 6.4

制备说明

R5503 的进一步色谱纯化得到的特纯 RNAse A

分析说明

蛋白测定方法:E.
通过 SDS-PAGE 测定 RNase A 纯度

基本信息

酶学委员会(EC)编号3.1.27.5 ( BRENDA | IUBMB )
MDL编号MFCD00132181
NACRESNA.54

产品性质

质量水平200
生物来源bovine pancreas
类型Type X-A
测定≥90% (SDS-PAGE)
形式buffered aqueous solution
specific activity≥70 Kunitz units/mg protein
分子量 ~13,700
异质活性protease, essentially free
储存温度−20℃

安全信息

象形图GHS08
警示用语:Danger
危险声明H334
预防措施声明P261 - P284 - P501
危险分类Resp. Sens. 1
储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves

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