4-Methylumbelliferyl N-acetyl-β-<sc>D</sc>-glucosaminide

产品介绍：

产品说明

应用

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide has been used as a fluorogenic substrate in the acidic chitinase activity assay. It has also been used to measure the total activity of β-N-acetylhexosaminidase (NAGase) in water sample filtrates.

生化/生理作用

4-Methylumbelliferyl-N-acetyl-β-D-glucosaminide (4-MUF-NAG) is a synthetic uncharged fluorogenic substrate for hexosaminidases. It consists of chitin monomer (N-acetyl-β-D-glucosaminide) and 4-methylumbelliferone (7-hydroxy-4-methylcoumarin) (4-MUF). 4-MUF-NAG is used for the detection and estimation of fungal growth by measuring the β-N-acetylhexosaminidase (NAGase) activity.

其他说明

β-N-乙酰氨基己糖苷酶的测定

基本信息

经验(实验)分子式	C₁₈H₂₁NO₈
分子量	379.36
Beilstein	1693397
EC 号	253-333-0
MDL编号	MFCD00150977
PubChem化学物质编号	57652199
NACRES	NA.32

产品性质

质量水平	100
测定	≥99.0% (TLC)
形式	powder
杂质	≤0.1% free 4-methylumbelliferone
溶解性	DMF: 20 mg/mL, clear, colorless
荧光	λ_ex 317 nm (pH 10.0) λ_ex 365 nm; λ_em 445 nm in aqueous buffer pH 5.0 (after cleavage by β-N-acetylglucosaminidase)
适用性	suitable for fluorescence
储存温度	−20℃
SMILES string	[H]O[H].[H]O[H].CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc3C(C)=CC(=O)Oc3c2
InChI	1S/C18H21NO8.2H2O/c1-8-5-14(22)26-12-6-10(3-4-11(8)12)25-18-15(19-9(2)21)17(24)16(23)13(7-20)27-18;;/h3-6,13,15-18,20,23-24H,7H2,1-2H3,(H,19,21);2*1H2/t13-,15-,16-,17-,18-;;/m1../s1
InChI key	PAVCYMSNMRWMAK-DMYIEBNJSA-N

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)

4-Methylumbelliferyl N-acetyl-β-D-glucosaminide

4-甲基伞形酮基 N-乙酰基-β- D -氨基葡萄糖