产品说明
一般描述
Tris(hydroxymethyl)aminomethane (Tris) is a routine buffer in biological sciences. The pH buffering range is between 7.0 -9.0. It displays negligible binding towards metal in solutions. Tris is used majorly in combination with ethylenediaminetetraacetic acid (EDTA), especially for electrophoretic studies. Its low ionic mobility finds usage in capillary electrochromatography as well.
一系列 TRIZMA 碱和 TRIZMA HCl 的预混合溶液,为 Tris 缓冲液提供常用的 pH 值。不必混合或调节 pH 值。保证精确度 ± 0.1 个 pH 单位。
应用
Trizma® hydrochloride solution has been used:
- as a component of lysis buffer for lysing human embryonic kidney (HEK) 293 cells
- in the 10X digoxigenin (DIG) deoxynucleoside triphosphate (dNTP) nick translation labeling buffer and in tris EDTA and sodium chloride (TEN) buffer
- in the preparation of tris, acetate, ethylenediaminetetraacetic acid (EDTA) (1x TAE) buffer and as a component of 10X gel loading dye
法律信息
Trizma is a registered trademark of sigma-aldrich Co. LLC
产品性质
质量水平 | 200 |
等级 | BioPerformance Certified for molecular biology |
形式 | solution |
浓度 | 1 M |
technique(s) | cell culture | mammalian: suitable |
杂质 | DNase, RNase, Protease, none detected bioburden, tested endotoxin, tested ≤5 ppm Heavy metals (as Pb) |
pH值(酸碱度) | 7.8 |
有效pH范围 | 7.0 - 9.0 |
吸光度 | ≤0.05 at 290 at 40% |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
个人防护装备 | Eyeshields, Gloves, type ABEK (EN14387) respirator filter |