产品说明
一般描述
Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.
应用
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.
LB Broth with agar (Luria low salt) has been used for culturing E. coli DH10B cells and in the isolation of diazotrophic bacteria.
特点和优势
Luria′s low salt LB powder with agar provides:
- Easy scale-up using larger package sizes
- A budget-friendly alternative to pre-poured plates
- Standard formulation
制备说明
1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121℃.
4. Cool to 50℃ prior to dispensing into petri dishes.
法律信息
Zeocin is a registered trademark of Cayla Sarl
产品性质
| 质量水平 | 200 |
| 等级 | for molecular biology |
| 无菌性 | non-sterile |
| 形式 | powder |
| 组成 | Agar, 15 g/L NaCl, 0.5 g/L Tryptone, 10 g/L Yeast Extract, 5 g/L |
| pH范围 | 6.8 - 7.2 (3.05% solution) |
| application(s) | food and beverages |
| 储存温度 | room temp |
| 适用性 | nonselective for Escherichia coli nonselective for coliforms |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
