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Gecko2 Mouse Whole Genome CRISPR Pool, gRNA Only Lenti Particles (Gecko2 vector)

品牌
Sigma-Aldrich
货号
MGECKO2G
包装型号
规格纯度
参考价格
89914.1 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Developed by Feng Zhang′s lab at the Broad Institute, the mouse GeCKO v2 (two vector system) libraries consist of over 100,000 unique gRNAs for gene knock-out in the mouse genome. Using a dual lenti CRISPR vector wherein the pooled libraries will only express the gRNA (with the lentiGuide-Puro vector), this system produces over 100X higher titer virus compared to version 1. The lentiGuide-Puro pool should be used only in cell lines with Cas9 already integrated (which can be generated using a separate lenti-Cas9-Blast vector). Sigma′s lentiviral mouse GeCKO pool guide RNA only is provided in 8 x 25 ul aliquots at a minimum titer of 5X10^8 TU/ml (measured by a p24 assay).
Each species-specific library is delivered as two half-libraries (A and B). It is recommended to screen both A and B libraries together, which will include 6 sgRNAs per gene (3 sgRNAs in each library). Both libraries contain 1000 non-targeting control sgRNAs. The A library also targets miRNAs (4 sgRNAs per miRNA).

应用

Functional Genomics/Screening /Target Validation

特点和优势

  • Use CRISPR nucleases to knockout protein-coding genes to assess their function
  • Efficiently screen the whole human genome (16,000+ genes) at the bench-top without robotics or specialized equipment
  • Numerous built-in enrichment and depletion controls allow researchers to confidently gauge the success of their pooled screening experiments • Lentiviral CRISPRs can infect a broad variety of mammalian cells by transducing a single guide RNA (sgRNA) to a Cas9-expressing mouse cell line to facilitate gene knockout for screening applications.
  • Use the dual vector system for the mouse GeCKO version 2 libraries for mouse cell lines that have Cas9 already integrated into the genome.
  • Use puromycin gRNA selection after transduction.

制备说明

Puro Kill Curve and Determining CFU (Colony Formation Unit) per mL. Prior to performing a library-scale screening, two preliminary experiments must be conducted. Visit Sigma.com/pooledscreening.

其他说明

该产品仅供研发使用,不可用作药物、家庭使用或其他用途。请查阅物质安全资料表,获取危险和安全操作规范信息。尽管生产的慢病毒转导颗粒是不可复制的,但仍然建议将其作为生物危害等级2级(RGL−2)生物体在实验室中进行处理。请遵循所有已发表的RGL-2指南,进行实验室废物处理和净化。

法律信息

CRISPR专利正在申请中

产品性质

质量水平200
包装pkg of 8x25 μL (vials)
浓度5x108  VP/ml (via p24 assay)
application(s)CRISPR
运输dry ice
储存温度−70℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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