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CMV-ESPCAS9-2A-RFP Plasmid

CMV-ESPCAS9-2A-RFP 质粒

品牌
Sigma-Aldrich
货号
ESPCAS9RFP
包装型号
规格纯度
参考价格
2486 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

This product is an expression plasmid that utilizes the CMV promoter for strong transient expression of eSpCas9 and RFP linked by a 2A peptide (CMV-eSpCas9-2A-RFP) allowing for easy visualization of successful transfection. The eSpCas9 expression plasmid is one part of a two part CRISPR system with individual eSpCas9 and gRNA expression vectors.

To order gRNA in any format click here

应用

Functional Genomics/Target Validation

  • Creation of gene knockouts in multiple cell lines
  • Complete knockout of genes not amenable to RNAi
  • Creation of knock-in cell lines with promoters, fusion tags, or reporters integrated into endogenous genes

特点和优势

  • Enhanced specificity compared to wild type Cas9
  • Highly active
  • Ready to use purified plasmid DNA

原理

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB. Newly engineered eSpCas9 enables the efficient targeted gene editing of established CRISPR systems with the benefit of reduced off-target effects. Point mutations in the chromosome-binding motif of SpCas9, as described by Slaymaker, et al., provide higher on-target fidelity without loss of cleavage efficiency.

法律信息

CRISPR Use License Agreement

Evrogen Fluorophore Licenses

产品性质

质量水平200
重组expressed in E. coli
包装vial of 50 μL
浓度20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
启动子Promoter name: CMV
报告基因RFP
selectionkanamycin
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 2
闪点(F)Not applicable
闪点(C)Not applicable

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