产品说明
一般描述
The MISSION pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer contain an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transduction of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.
High titer virus is useful when performing in vivo research.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results. The MISSION Control Transduction Particles are a critical positive control to monitor transduction efficiency.
To see more application data, protocols, vector maps visit sigma.com/shrna.
应用
To see more application data, protocols, vector maps visit sigma.com/shrna.
法律信息
MISSION is a registered trademark of sigma-aldrich Co. LLC
基本信息
NACRES | NA.51 |
产品性质
质量水平 | 200 |
产品线 | MISSION® |
浓度 | ≥1x109 VP/ml (via p24 assay) |
运输 | dry ice |
储存温度 | −70℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 3 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |