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Anti-Vimentin antibody produced in goat

品牌
Sigma-Aldrich
货号
V4630
规格纯度
whole antiserum
参考价格
5827.44 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The VIM (vimentin) gene codes for an intermediate filament protein, that is expressed throughout mesenchymal cells. VIM gene is mapped to human chromosome 10p13.
Vimentin is encoded by a single-copy gene mapped to human chromosome 10p13. 53kDa vimentin protein belongs to type III intermediate filament (IF) family and is specifically expressed in normal mesenchymal cells. Vimentin protein with 466 amino acids is characterized with a highly conserved α-helical “rod” domain that is flanked by non-α-helical amino- terminal “head” and carboxy-terminal “tail” domain.

特异性

The antibody localizes vimentin in normal and pathological tissue of mesenchymal derivation. The antibody shows wide cross-reactivity among mammalian species.

免疫原

vimentin from cultured human foreskin fibroblasts.

应用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Immunocytochemistry (1 paper)
Immunofluorescence (1 paper)
Immunohistochemistry (1 paper)
Anti-Vimentin antibody produced in goat has been used in Immunohistochemistry and Western blot analysis.
Anti-Vimentin antibody produced in goat has been used in following studies:

  • immunohistochemistry
  • western blotting
  • immunocytochemistry
  • flow cytometry

生化/生理作用

Vimentin is known to promote epithelial to mesenchymal transition and contributes to metastasis in cancer such as ovarian cancer. Vimentin preserves cellular structure and tissue integrity. Vimentin is also known to bring about TNF-α (tumor necrosis factor-α) induced cell apoptosis.
Vimentin plays a vital role in maintaining the structural integrity of the cells. Overexpression of the gene has been observed in prostate cancer, breast cancer, endometrial cancer, central nervous system (CNS) tumors, malignant melanoma and gastrointestinal tumors including pancreatic, colorectal and hepatic cancers. Thus, vimentin can act as a target for developing therapeutics for treating these cancers. Vimentin plays a crucial role in the vascular endothelial growth factor receptor-1 (VEGFR-1) induced activation of the protein kinase G (PKG) 1 signaling pathway, stimulating regression of cardiomyocyte hypertrophy. Vimentin filaments is implicated in various physiological process including migration, maintenance of cell shape and tolerance of mechanical stress of mesenchymal cells. The protein interacts with LARP6 (la ribonucleoprotein domain family member 6) and stabilizes type I collagen mRNAs and might play a vital role in the development of tissue fibrosis. Vimentin plays an essential role in the maintenance of lens integrity, therefore mutations in the gene causes dominant and pulverulent cataract.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号MFCD00145922

产品性质

质量水平200
生物来源goat
偶联物unconjugated
抗体形式whole antiserum
antibody product typeprimary antibodies
克隆polyclonal
分子量antigen 58 kDa
包含15 mM sodium azide
species reactivityhuman, wide range
technique(s)immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
indirect immunofluorescence: 1:20 using formalin-fixed, paraffin-embedded sections of human tissue
western blot: 1:50 using HS-68 human foreskin cell extract
UniProt登记号P08670
运输dry ice
储存温度−20℃
Gene Informationhuman ... VIM(7431)

安全信息

储存分类代码13 - Non Combustible Solids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable
个人防护装备Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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