产品说明
一般描述
Anti-Actin (Ab-1), mouse monoclonal, clone JLA20, recognizes the ~ 42 kDa α-, β-, and γ isoforms of actin. It is validated for Western blotting and immunofluorescence.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.
Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells supplied as ascites. Recognizes the ~42 kDa α-, β- and γ-isoforms of actin. Supplied with goat anti-mouse IgM conjugated to horseradish peroxidase.
免疫原
Chicken
chicken cytoskeletal proteins
包装
1 ea in Plastic ampoule
应用
Immunoblotting (1:5000-1:20000)
Immunofluorescence (1:10000, see application references)
Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
警告
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
外形
100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
重悬
Following initial thaw, aliquot ascites and freeze (-20°C).
分析说明
Positive Control
All animal cell lines
其他说明
Lin, J.J. et al. 1984 in Monoclonal antibodies and functional cell lines. Plenum Publishing Corp.
Lin, J.J. 1982. Cold Spring Harbor Symp. on Quant. Biol.46, 769.
Lin, J.J. 1981. Proc. Natl. Acad. Sci.78, 2335.
Goldman, R.D., et al. 1979. Ann. Rev. Physiol.41, 701.
Goldman, R., et al. (eds.) 1976. Cold Spring Harbor Conference on Cell Proliferation, Books A-C.
Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
| 质量水平 | 100 |
| 生物来源 | mouse |
| 抗体形式 | ascites fluid |
| antibody product type | primary antibodies |
| 克隆 | JLA20, monoclonal |
| 形式 | liquid |
| 包含 | ≤0.1% sodium azide as preservative (actin antibody only, CP01-100UL) |
| species reactivity (predicted by homology) | all |
| manufacturer/tradename | Calbiochem® |
| 储存条件 | OK to freeze |
| 同位素/亚型 | IgM |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
