产品说明
一般描述
This product corresponds to ATCC Cat. No. CCL-231
应用
Media Renewal changes one to two times per week
The cell freezing medium is DMSO 1X (Cat. No. C6164)
The base medium for this cell line is DMEM, Cat. No. D5671. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration (v/v) of 10%, Sodium pyruvate, Cat. No. S8636, at a final concentration of 1 mM, and L-glutamine, Cat. No. G7513, at a final concentration of 2mM.
Subculture Ratio: approx. 1:2 to 1:6
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.
特点和优势
These SW48 cells are adherent, with a doubling time of approx. 35 hours.
Zinc finger nuclease (ZFN) double knock out on chromosome 19q13.3-13.4 / 6p21.3
质量
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.
产品性质
| 质量水平 | 200 |
| 生物来源 | human female colon (Source Disease: Colorectal adenocarcinoma) |
| OMIM登记号 | 600040 / 600516 |
| 储存温度 | −196℃ |
| Gene Information | human ... BAK(578), BAX(581) |
组份列表
组份可单独销售
| 货号 | 组份 |
| SW48 Cells BAX/BAK (-/-,-/-) 1 vial | |
| SW48 Control Cells 1 vial |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
