产品说明
一般描述
This particular protease inhibitor cocktail omits chelators (e.g., EDTA), to optimize its use for purification of histidine-tagged proteins.1 It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from E. coli and insect cells This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0004 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8849.
特点和优势
- Does not freeze in storage for quick, convenient use.
- Tailored for use with Histidine fusion tagged proteins.
- Suggested as 100 X solution.
- Reclosable screw-top vials.
- Can be stored in cooler for approx. 1 month or room temperature for a week.
制备说明
The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 20 g (wet weight) of E. coli cells or 10 g (wet weight) of baculovirus-infected insect (Spodoptera frugiperda) cells.
其他说明
For life science research only. Not for use in diagnostic procedures.
法律信息
ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany
产品性质
| 描述 | Purification of Histidine-Tagged Proteins |
| 形式 | (non-freezing liquid formulation ) |
| 质量 | 200 |
| 包装 | pkg of 1 mL (in amber polypropylene vial) pkg of 5 mL (in amber polypropylene vial) |
| web metadata keyword.default | Extracts, Lysate, Cell Extracts, Tissue Extracts, Mammalian Tissue Extract, Bacterial lysate, Recombinant extracts, fusion proteins, Electrophoresis, peptidase inhibitor, proteinase inhibitor |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
