产品说明

一般描述

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute^™ Mammalian Total RNA Miniprep Kit and the Spectrum Plant Total RNA Kit. It can also be used with many other commercially available RNA purification kits.
通过下文命名的 Sigma 试剂盒标准方案中提供的程序，将绝大多数 DNA 从总 RNA 纯化中去除。然而，对于非常灵敏的应用，如定量 RT-PCR，即使微量的 DNA 也会导致 cDNA 来源扩增子的假阳性检测。
柱上 DNase I 消化套装为从总 RNA 制备物中去除基因组 DNA 提供了一种简单方便的方法。本套装拟与 GenElute ^™ Mammalian Total RNA Miniprep Kit、GenElute Bacterial Total RNA Purification Kit、GenElute Yeast Total RNA Purification Kit 以及 Spectrum^™ Plant Total RNA Kit配套使用。不过酶切方案与许多其他市售的 RNA 纯化试剂盒兼容。
术语“柱上”表示消化是在过程中完成的，以确保方便。Sigma的每个总RNA纯化试剂盒都使用结合柱进行纯化。 DNase I消化在15分钟内完成，同时总RNA与柱底物结合。 随后立即进行几次洗涤步骤和总RNA的洗脱。 要查看完整的方案，请参阅上面列出的任何工具包的技术公告。

应用

On-Column DNase I Digestion Set is suitable for removing gDNA from total RNA preparations. It may not be suitable for RT-PCR applications as a small amount of residual DNA may remain. On-Column DNase I Digestion Set was used to purify RNA preparations isolated from Vitis vinifera cultivars.

特点和优势

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute^™ Mammalian Total RNA Miniprep Kit, the GenElute Bacterial Total RNA Purification Kit, the GenElute Yeast Total RNA Purification Kit and the Spectrum^™ Plant Total RNA Kit. However, the digestion protocol is compatible with many other commercially available RNA purification kits.

原理

The on-column digestion is accomplished in-process for convenience. DNase I digestion is accomplished in 15 minutes while total RNA is bound to the column substrate. Several wash steps and elution of the total RNA immediately follow.

法律信息

GenElute is a trademark of sigma-aldrich Co. LLC
Spectrum is a trademark of sigma-aldrich Co. LLC

基本信息

产品性质

安全信息