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Nitrite Assay Kit (Griess Reagent)

品牌
Sigma-Aldrich
货号
MAK367
规格纯度
sufficient for 200 colorimetric tests
参考价格
2056.39 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Nitrogen-based ions, nitrite and nitrate, are found in almost every living organism. Furthermore, endogenous Nitrite levels are found in mammals and can also be obtained from dietary sources. In humans, nitrite is further metabolized to Nitric Oxide and other reactive nitrogen species (nitrogen oxides). The Nitrate-Nitrite-NO biochemical pathway is well known for its participation in cell signaling, hypoxiadependent response and regulation of blood flow. Recent studies suggest the reduction of Nitrite to Nitrogen Oxygen in the mitochondria. Specifically, myoglobin and xanthine oxidoreductase could generate NO under hypoxic conditions leading to mitochondrial respiration.

适用性

The kit is suitable for the detection of Nitric Oxide in animal tissues (liver, kidney, etc.), cell culture (adherent or suspension cells), serum, plasma and urine.

原理

The Nitrite Assay Kit utilizes the Griess Reagent, a classic protocol for the estimation of nitrite. In the assay, nitrite is reduced to Nitrogen Oxide using Griess Reagent I. Then, Nitrogen Oxide reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at A540 nm. The one-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.

产品性质

用途 sufficient for 200 colorimetric tests
detection methodcolorimetric
储存温度2-8℃

安全信息

象形图GHS05
警示用语:Danger
危险声明H290 - H314
预防措施声明P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
危险分类Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
储存分类代码8A - Combustible, corrosive hazardous materials
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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