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Lipolysis (Adipocyte) Kit

脂解(脂肪细胞)检测试剂盒

品牌
Sigma-Aldrich
货号
MAK195
规格纯度
Sufficient for 5 g of tissue
参考价格
8205.05 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Lipolysis is the process of hydrolyzing triglycerides to free fatty acids and glycerol. This process involves the action of adipose TG lipase (ATGL), hormone-sensitive lipase (HSL), and monoglyceride lipase. Lipolysis maintains the energy balance during fasting and exercise by providing a substrate for oxidative metabolism. Lipolysis is regulated by nutritional factors and hormones. Problems with the regulation of lipolysis are associated with obesity, diabetes, and metabolic syndromes.

适用性

该试剂盒适用于从组织中分离脂肪细胞和脂解作用的测定。

原理

The Lipolysis (Adipocyte) Kit provides contains synthetic catecholamine (isoproterenol) that activates β-adrenergic receptors. This results in the activation of adenylate cyclase that converts ATP to cAMP. cAMP then activates the hydrolysis of triglycerides by hormone-sensitive lipase. Lipolysis is determined by measuring a fluorescent (λex = 535/ λem = 587 nm) or colorimetric (570 nm) product proportional to the amount of glycerol present.

基本信息

EC 号259-322-7
NACRESNA.25

产品性质

detection methodcolorimetric
fluorometric
储存温度−20℃

组份列表

组份不可单独销售

货号组份
Collagenase (0.2%)
Collagenase Stop Buffer
Adipocyte Wash Buffer
Adipocyte Lipolysis Buffer
Glycerol Assay Buffer
Glycerol Probe, in DMSO
Glycerol Enzyme Mix
Glycerol Standard, 100 mM
Isoproterenol, 10 mM
Cell Strainer

安全信息

象形图GHS07
警示用语:Warning
危险声明H315 - H319 - H335
预防措施声明P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
危险分类Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
靶器官Respiratory system
储存分类代码10 - Combustible liquids
WGKWGK 3

Sigma-Aldrich

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