产品说明
一般描述
The Streptavidin IA-MS Immunoaffinity Mass Spectrometry Kit is a 96-well platform enabling robust, high-throughput quantification of proteins in complex backgrounds by LC-MS/MS. Users provide their own biotinylated capture molecule and internal standard (available separately, internal standards may be SIL peptides or preferably full length SIL protein) and the kit contains all other components needed for in-well enrichment and rapid trypsin digestion of samples. The plate format consists of 8-well strips allowing for partial runs, and avoids the high cost and potential LC column blockage associated with bead-based enrichment formats. The entire workflow can be performed in under 5 hours, resulting in digests ready for injection with no SPE or other cleanup required.
应用
Target protein enrichment and quantification from complex background by mass spectrometry.
特点和优势
- Entire workflow including digest completed in under 5 hours, such that samples are injection ready at the end of the work day
- 96-well plate format has potential for automation in a liquid-handling system
- Bead-free system circumvents messy clean-up and eliminates risk of column clogging
- Plate strips allow user to run as many or as few samples as desired
包装
Box with foam insert containing kit components.
组分
Streptavidin High Binding Capacity Coated Plate S2577-1EA
SOLu-Trypsin EMS0004-100UG
MS Denaturation Solution EMS00010-10ML
Rapid Trypsin Digestion Buffer EMS0009-30ML
Tris Buffered Saline with TWEEN® 20 (TBST)powder, pH 8.0 T9039
Tris Buffered Saline (TBS) powder, pH 8.0 T6664
EZ-Pierce™ plate seal Z721581-1EA
See datasheet for more information.
储存及稳定性
2 years when stored at 2-8 ℃.
Related Presentation
- Development of a Plate-based Immunoaffinity LC-MS/MS Workflow for Sensitive Quantitation of Free Infliximab and Adalimumab in Human Serum (Poster, PDF)
法律信息
EZ-Pierce is a trademark of Excel Scientific, Inc.
TWEEN is a registered trademark of Croda International PLC
产品性质
| 形式 | solid |
| 适用性 | suitable for mass spectrometry |
| 运输 | wet ice |
| 储存温度 | 2-8℃ |
组份列表
组份不可单独销售
| 货号 | 组份 |
| Rapid Trypsin Digestion Buffer 30 mL | |
| MS Denaturation Solution |
安全信息
| 象形图 |    |
| 警示用语： | Danger |
| 危险声明 | H226 - H301 + H311 + H331 - H370 |
| 预防措施声明 | P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311 |
| 危险分类 | Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 3 - STOT SE 1 |
| 储存分类代码 | 3 - Flammable liquids |
| WGK | WGK 3 |
| 闪点(F) | 75.2 °F |
| 闪点(C) | 24 ℃ |
