产品说明
一般描述
HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG MDR3 knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.
应用
See technical bulletin for detailed protocols
特点和优势
Zinc finger nucleases (ZFN) mediated Knockout of ABCB11 (MDR3) gene.
- The frame-shift mutation of ABCB11 gene was confirmed by fragment length analysis and DNA sequencing.
- Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay.
HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity. - Sigma′s HepaRG MDR3 Knockout (KO) allows investigations of drug-transporter interactions involving MDR3 and Cholestasis in the liver.
质量
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
法律信息
Exhibit 1: ADME/Tox cell lines license
Exhibit 2: HepaRG limited use license
HepaRG is a trademark of BioPredic International company
基本信息
| NACRES | NA.81 |
产品性质
| 质量水平 | 200 |
| 生物来源 | human female liver (hepatocarcinoma and hepatitis C tumor) |
| 形式 | liquid |
| OMIM登记号 | 603201 |
| Gene Information | human ... ABCB11(8647) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
