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Granzyme B Activity Assay Kit

颗粒酶B活性检测试剂盒

品牌
Sigma-Aldrich
货号
MAK176
规格纯度
Sufficient for 100 Fluorometric tests
参考价格
7329.47 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

GZMB codes for granzyme B.
Granzyme B (gzm B) is a peptidase that induces cytotoxic T lymphocyte (CTL)-mediated DNA fragmentation and apoptosis in allogeneic target cells. It cleaves after aspartic acid residues in substrates. Granzyme B-mediated release of mitochondrial cell death factors are required for the activation of caspase-3. Furthermore, the degradation of granzyme B is known to decrease the susceptibility of cancer cells to NK-cell-mediated lysis during hypoxia. Granzyme B activity assays can be used for studying T cell immunity, CTL responses, cell-mediated cytotoxicity, and apoptosis.
Gzm B is a pro-apoptotic member of the granzyme family. It is a neutral serine protease with Aspase activity. Gzm B is expressed exclusively in the granules of activated cytolytic T cells, natural killer cells and lymphokine-activated killer cells.

适用性

适于检测生物样本中的颗粒酶B活性和筛选颗粒酶B的抑制剂。

原理

Granzyme B activity is determined using an enzyme-catalyzed reaction in which the non-fluorescent substrate, Ac-IEPD-AFC, is hydrolyzed resulting in the release of AFC (λex = 380/λem = 500 nm) proportional to the enzymatic activity present. One unit of granzyme B is the amount of enzyme that hydrolyzes 1 pmole of the substrate per minute at 37 ℃.

基本信息

EC 号200-664-3
NACRESNA.84

产品性质

用途 sufficient for 100 fluorometric tests
detection methodfluorometric
储存温度−20℃
Gene Informationhuman ... GZMB(3002)
mouse ... GZMB(14939)
rat ... GZMB(171528)

组份列表

组份不可单独销售

货号组份
Granzyme B Assay Buffer
Granzyme B Substrate
Granzyme B Positive Control
AFC Standard, 1 mM

安全信息

储存分类代码10 - Combustible liquids

Sigma-Aldrich

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