产品说明
一般描述
Gelatinases are a type of matrix zinc-dependent metalloproteases (MMPs) that degrade gelatins and a variety of other extracellular matrix proteins. These enzymes are synthesized as latent zymogens that are activated by proteolysis and inhibited by tissue inhibitors of metalloproteases (TIMPs). Two mammalian gelatinases, Gelatinase A (MMP-2) and Gelatinase B (MMP-9), are critical for basement membrane degradation and are highly upregulated in variety of tumor cells.
特点和优势
Compatible with high-throughput handling systems.
适用性
Suitable for the detection of gelatinase activity in biological samples such as recombinant protein, tissue, cell lysates, etc
原理
The Gelatinase Activity Assay Kit utilizes a hybrid approach for the detection of gelatinase activity by employing a highly quenched gelatin substrate which upon cleavage by a suitable gelatinase releases a fluorophore, which can be easily quantified using a conventional microplate reader. This method is substrate-specific, simple, fast, high-throughput adaptable and amenable to the sensitive detection of gelatinase activity (as low as 0.06 mCDU).
产品性质
用途 | sufficient for 100 fluorometric tests |
detection method | fluorometric |
储存温度 | −20℃ |
安全信息
危险声明 | H412 |
预防措施声明 | P273 - P501 |
危险分类 | Aquatic Chronic 3 |
储存分类代码 | 10 - Combustible liquids |
WGK | WGK 3 |
闪点(F) | 188.6 °F |
闪点(C) | 87 ℃ |