产品说明
一般描述
Cholesterol is an essential molecule in all animal life. It has been involved in both normal development and diseases. The Cholesterol/Cholesteryl Ester Quantitation Kit provides a simple method for sensitive quantification of free cholesterol, cholesteryl esters, or both using a colorimetric method. Most of the cholesterol in blood is in the form of cholesteryl ester. These esters can be hydrolyzed to free cholesterol under the appropriate conditions.
特点和优势
Cholesterol/Cholesteryl Ester Kit II:
- Simple procedure with results in ∼1 hour.
适用性
The kit is suitable for the quantification of cholesterol/cholesteryl ester in serum, cells and tissue samples.
原理
In the assay, free cholesterol is oxidized by cholesterol dehydrogenase to generate NADH which reacts with a sensitive probe resulting in strong absorbance at A450 nm. The assay can detect free or total cholesterol depending upon whether esterase is utilized to hydrolyze cholesterol esters present. Cholesteryl ester can be determined by subtracting the value of free cholesterol from the total cholesterol (cholesterol plus cholesteryl esters). The probe in the kit is more stable, sensitive and specific. The assay can tolerate interferences from various samples significantly.
产品性质
质量水平 | 100 |
web metadata keyword.default | Cholesterol/Cholesteryl Ester Kit II|sigma-aldrich |
detection method | colorimetric |
储存温度 | −20℃ |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H314 |
预防措施声明 | P280 - P301 + P330 + P331 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 |
危险分类 | Eye Dam. 1 - Skin Corr. 1B |
储存分类代码 | 8A - Combustible, corrosive hazardous materials |