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Cathepsin L Activity Assay Kit

品牌
Sigma-Aldrich
货号
MAK401
规格纯度
sufficient for 100 fluorometric tests
参考价格
6019.62 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.

特点和优势

Cathepsin L Activity Assay Kit:

  • Simple procedure with results in 1-2 hours.

适用性

The kit is suitable for the measurement of cathepsin L in cell lysates.

原理

The Cathepsin L Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin L substrate sequence FR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin L will cleave the synthetic substrate FR-AFC to release free AFC. The released AFC can easily be quantified fluorometrically. The cathepsin L assay is simple and straightforward. Assay conditions have been optimized to obtain the maximal activity.

产品性质

质量水平100
web metadata keyword.defaultCathepsin L Activity Assay Kit|sigma-aldrich
detection methodfluorometric
储存温度−20℃

安全信息

象形图GHS05GHS08
警示用语:Danger
危险声明H315 - H318 - H334
预防措施声明P261 - P264 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
危险分类Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2
储存分类代码10 - Combustible liquids

Sigma-Aldrich

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