产品说明
一般描述
Apoptosis can be mediated by mechanisms other than the traditional caspase-mediated cleavage cascade. There is growing recognition that alternative proteolytic enzymes such as the lysosomal cathepsin proteases may initiate or propagate proapoptotic signals. Cathepsins are lysosomal enzymes that are also used as sensitive markers in various toxicological investigations.
特点和优势
Cathepsin L Activity Assay Kit:
- Simple procedure with results in 1-2 hours.
适用性
The kit is suitable for the measurement of cathepsin L in cell lysates.
原理
The Cathepsin L Activity Assay kit is a fluorescence-based assay that utilizes the preferred cathepsin L substrate sequence FR labeled with AFC (amino-4-trifluoromethyl coumarin). Cell lysates or other samples that contain cathepsin L will cleave the synthetic substrate FR-AFC to release free AFC. The released AFC can easily be quantified fluorometrically. The cathepsin L assay is simple and straightforward. Assay conditions have been optimized to obtain the maximal activity.
产品性质
质量水平 | 100 |
web metadata keyword.default | Cathepsin L Activity Assay Kit|sigma-aldrich |
detection method | fluorometric |
储存温度 | −20℃ |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H315 - H318 - H334 |
预防措施声明 | P261 - P264 - P280 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 |
危险分类 | Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 |
储存分类代码 | 10 - Combustible liquids |