产品说明
一般描述
The C-Peptide Enzyme Immunoassay (EIA) Kit is an in vitro quantitative assay for detecting C-Peptide peptide based on the principle of Competitive Enzyme Immunoassay. EIA-CPE-1 only detects C-peptide, not Insulin A or B chain.
免疫原
C-Peptide , synthetic peptide
应用
请参考Protocol了解详情。
生化/生理作用
C-peptide inhibits endothelial dysfunction under high glucose conditions via inhibition of nuclear factor-κB (NF-κB) pathway. Experimental studies suggest that prolonged dosage of physiological concentration of C-peptide to type 1 diabetes patients minimizes diabetes-induced complications, such as glomerular hypertrophy and mesangial matrix expansion by interrupting transforming growth factor β1 (TGF-β1) and tumor necrosis factor α (TNFα) signaling pathway. C-peptide reduces diabetes-induced glomerular hyperfiltration by contraction of the afferent arteriole, dilation of the efferent arteriole, and by inhibition of tubular reabsorption. C-Peptide also plays a crucial role in renal pathophysiology. C-peptide binds to a cell surface receptor that is likely to be G-protein coupled. This interaction stimulates various cellular functions such as increasing intracellular Ca2+ concentration, enhancing PI3-K (phosphoinositide 3-kinase) activity, increased endothelial nitric oxide synthase (eNOS) transcription, and activation of the Na+/K+ ATPase and mitogen-activated protein kinase (MAPK) signaling pathway.
基本信息
| NACRES | NA.32 |
产品性质
| species reactivity | human, rat, mouse |
| 包装 | kit of 96 wells (12 strips x 8 wells) |
| technique(s) | ELISA: suitable enzyme immunoassay: suitable |
| input | sample type cell lysate sample type culture supernatant(s) sample type plasma sample type serum |
| assay range | inter-assay cv: <15% intra-assay cv: <10% sensitivity: 772 pg/mL standard curve range: 0.1-1000 ng/mL |
| detection method | colorimetric |
| 运输 | wet ice |
| 储存温度 | −20℃ |
| Gene Information | human ... INS(3630) |
组份列表
组份可单独销售
| 货号 | 组份 |
| RABHRP3 | HRP-Streptavidin |
安全信息
| 象形图 |  |
| 警示用语： | Warning |
| 危险声明 | H290 |
| 预防措施声明 | P234 - P390 |
| 危险分类 | Met. Corr. 1 |
| 储存分类代码 | 8A - Combustible, corrosive hazardous materials |
| WGK | WGK 3 |
