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U2OS GFP-TUBA1B

品牌
Sigma-Aldrich
货号
CLL1031
包装型号
规格纯度
参考价格
59056.1 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

This product corresponds to ATCC Cat. No. HTB-96.
U2OS GFP-TUBA1B are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with a GFP-tagged modification.
K-α1 tubulin/ tubulin α 1b (TUBA1B) is a microtubule protein. It is a ~50 kDa protein in its poly glycosylated, post-translational form. TUBA1B is present in cells as one of the six different isotypes. This gene is located on human chromosome 12q13.12.

应用

This product is a human U2OS cell line in which the genomic TUBA1B gene has been endogenously tagged with a Green Fluorescent Protein (GFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of the fusion protein in which GFP is attached to the N-terminus of tubulin. Fluorescence imaging shows that the tubulin can polymerize to form the characteristic pattern of microtubules. Addition of vincristine to this cell line shows the expected depolymerization of microtubules. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage

生化/生理作用

K-α1 tubulin/ tubulin α 1b (TUBA1B) participates in several cellular activities like GTP binding, GTPase activity, maintaining cellular structure in the form of microtubules and microtubule-based intracellular movement. It plays a crucial role in polymerization and depolymerization during cell replication and division. TUBA1B is overexpressed in hepatocellular carcinoma (HCC) tumor tissues and proliferating HCC cells.
Tubulin α 1b (TUBA1B) plays an important role in dynamic process of polymerization and depolymerization during cell replication and division. Elevated expression of the gene has been observed in hepatocellular carcinoma (HCC) tumor tissues and proliferating HCC cells. This upregulated expression of the gene leads to poor prognosis and chemotherapy resistance in HCC.

特点和优势

GFP-tagged on chromosome 12q13.12
The U2OS cells are adherent, with a doubling time of approx. 29 hours.

组分

U2OS GFP-TUBA1B cells

质量

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, PCR assay for cell line species confirmation.

制备说明

Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164).

法律信息

CompoZr is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRESNA.81

产品性质

质量水平200
生物来源human female bone (Source Disease: Osteosarcoma)
OMIM登记号602530
储存温度−196℃
Gene Informationhuman ... alpha tubulin 1B(10376)

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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