产品说明
一般描述
This product was derived from ATCC Cat. No. HTB-96.
U2OS BFP-LMNB1 RFP-ACTB are osteosarcoma, epithelial cells, from a human caucasian female (aged 15 years), with BFP-tagged and RFP-tagged modifications.
应用
This product is a human U2OS cell line in which the genomic LaminB1 gene has been endogenously tagged with a Blue Fluorescent Protein (BFP) and the genomic ACTB gene has been endogenously tagged with a Red Fluorescent Protein (RFP) gene using CompoZr® Zinc Finger Nuclease technology. Integration resulted in endogenous expression of a fusion protein in which BFP is attached to the N-terminus of LaminB1 and of a fusion protein in which RFP is attached to the N-terminus of actin. Fluorescence imaging shows the characteristic LaminB1 nuclear expression and that the actin can polymerize to form the characteristic pattern of actin filaments. This stable cell line was expanded from a single clone. The target′s gene regulation and corresponding protein function are preserved in contrast to cell lines with overexpression via an exogenous promoter.
To learn more, please visit the Cellular Reporter Cell Line webpage
特点和优势
LMNB1 is BFP-tagged on chromosome 5q23.3-q31.1 and ACTB is RFP-tagged on chromosome 7p22.1.
U2OS cells are adherent, with a doubling time of approx. 29 hours.
组分
U2OS BFP-LMNB1 RFP-ACTB cells
质量
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, and PCR assay for cell line species confirmation.
制备说明
Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37℃ in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1:3-1:6
The base medium for this cell line is McCoy′s 5A1 Medium Modified, Cat. No. M9309. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Cat. No. F4135, to a final concentration of 10%.
The cell freezing medium is DMSO 1X (Cat. No. C6164).
法律信息
CompoZr is a registered trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.81 |
产品性质
| 质量水平 | 200 |
| 生物来源 | human female bone (Disease source: Osteosarcoma) |
| OMIM登记号 | 102630 150340 |
| 储存温度 | −196℃ |
| Gene Information | human ... ACTB(60), LMNB1(4001) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
