产品说明
一般描述
Tyrosinase is a copper-binding enzyme that is expressed across a vast range of species ranging from bacteria and fungi to mammals. It is involved in two sequential reactions of the melanin synthesis pathway; first being the hydroxylation of a monophenol and second the conversion of an ortho-diphenol to a quinone. Quinone then undergoes a series of reactions including polymerization to form melanin. Tyrosinase is of great interest to the agriculture industry since it causes browning of fruits, vegetable and mushrooms, as well as to the cosmetic industry as it causes skin darkening. Development and screening of tyrosinase inhibitors, therefore, is very useful for conditions such as hyperpigmentation and melasma. Tyrosinase activity is significantly increased in melanoma. Therefore, the detection of tyrosinase activity could be promising as a specific diagnostic test for melanoma and may be useful in monitoring patient response to melanoma treatments.
特点和优势
Tyrosinase Activity Assay Kit:
- Non-radioactive assay.
- Sensitive, detecting Tyrosinase Activity ≤30 μU.
适用性
The kit is suitable for the measurement of tyrosinase activity in cell lysates (e.g. Melanoma cells), tissue lysates, plant tissue lysates (e.g. potato), recombinant enzyme and purified protein.
原理
In this assay, tyrosinase catalyzes the conversion of a phenolic substrate to a Quinone intermediate, which reacts with the tyrosine enhancer forming a highly stable chromophore with absorbance at A510 nm. The assay can detect as low as 30 μU Tyrosinase in biological samples.
产品性质
质量水平 | 100 |
web metadata keyword.default | Tyrosinase Activity Assay Kit (Colorimetric)|sigma-aldrich |
detection method | colorimetric |
储存温度 | −20℃ |
安全信息
象形图 | |
警示用语: | Danger |
危险声明 | H301 - H315 - H319 - H335 |
预防措施声明 | P261 - P264 - P270 - P301 + P310 - P302 + P352 - P305 + P351 + P338 |
危险分类 | Acute Tox. 3 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3 |
靶器官 | Respiratory system |
储存分类代码 | 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects |