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Reduced Glutathione (GSH) Assay Kit (Colorimetric)

品牌
Sigma-Aldrich
货号
MAK364
规格纯度
sufficient for 100 colorimetric tests
参考价格
6597.4 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

Glutathione (GSH), a thiol-containing tripeptide (γ-glutamyl-cysteinyl-glycine), is a key antioxidant in many species. It has been highly implicated in the detoxification/elimination of xenobiotics (naturally occurring harmful compounds such as free radicals, hydroperoxides etc.) and in the maintenance of the oxidation state of protein sulfhydryl groups. In addition, GSH plays a pivotal role in the pathogenesis of numerous human diseases including cancer and cardio-vascular diseases. Glutathione is present in cells in both reduced (GSH) and oxidized (GSSG) forms- GSH being, the predominant species under normal physiological conditions inside cells. Thus, pathologic conditions causing oxidative stress would result in increased levels of GSSG. Therefore, the measurement of intracellular GSH appears to be a sensitive indicator of the overall cell health, and its ability of resisting toxic challenges.

适用性

The kit is suitable for the measurement of reduced glutathione in various biological samples/preparations including tissue homogenates (liver, etc.) and cell lysates (Hep G2, Jurkat, etc.).

原理

The Reduced Glutathione (GSH) Assay Kit is based on an enzymatic cycling method in the presence of GSH and a chromophore. The reduction of the chromophore produces a stable product, which can be followed kinetically at A450 nm. Therefore, its absorbance is directly proportional to the amount of GSH in the sample. The kit is highly specific and sensitive because GSSG does not interfere with the assay. The kit includes 5-Sulfosalicylic acid (SSA) in order to protect samples′ GSH endogenous content. (SSA acts as a deproteinizing and antioxidant agent. The assay is simple, reproducible and can specifically detect as low as 50 pmol/well of reduced form of Glutathione (GSH) in a 100 μL reaction.

产品性质

用途 sufficient for 100 colorimetric tests
detection methodcolorimetric
储存温度−20℃

安全信息

象形图GHS05GHS08
警示用语:Danger
危险声明H314 - H317 - H334 - H341
预防措施声明P202 - P261 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
危险分类Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
储存分类代码8A - Combustible, corrosive hazardous materials
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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