Phosphoenolpyruvate Carboxykinase Activity Assay Kit (Colorimetric)

产品说明

一般描述

Phosphoenolpyruvate Carboxykinase (PEPCK) is an enzyme which belongs to the lyase family. In the presence of GTP, it catalyzes the reversible conversion of oxaloacetate (OAA) into phosphoenolpyruvate (PEP), GDP and CO2. In humans, two isoforms of PEPCK are found: cytosolic form (PEPCK-C, also called PCK1) and mitochondrial form (PEPCK-M). PEPCK-C is a rate-controlling step in gluconeogenesis. Recent studies found abnormal concentrations of PEPCK in diabetic mice. Therefore, accurate measurement of Phosphoenolpyruvate Carboxykinase activity is valuable for both mechanistic and therapeutic studies.

特点和优势

Phosphoenolpyruvate Carboxykinase Activity Assay Kit:

• Compatible with high-throughput handling systems.
• Sensitive, detecting than 10 μU of Phosphoenolpyruvate Carboxykinase activity per sample.

适用性

The kit is suitable for the measurement of Phosphoenolpyruvate Carboxykinase activity in various samples including tissue (rat liver, kidney, heart etc.) and adherent/suspension cells (HeLa, Jurkat, HEK 293 cells etc.). As well as the analysis of the gluconeogenesis pathway.

原理

In this assay, Phosphoenolpyruvate Carboxykinase is coupled with a set of enzymes that convert phosphoenolpyruvate and carbonate into a series of intermediates and hydrogen peroxide, which in turn, reacts with a probe and is converted generating a colorimetric signal (A₅₇₀ nm). The color intensity is directly proportional to the amount of active Phosphoenolpyruvate Carboxykinase present in samples.

产品性质

安全信息

象形图
警示用语：	Danger
危险声明	H317 - H334 - H335
预防措施声明	P261 - P271 - P272 - P280 - P284 - P302 + P352
危险分类	Resp. Sens. 1 - Skin Sens. 1 - STOT SE 3
靶器官	Respiratory system
储存分类代码	10 - Combustible liquids
WGK	WGK 3