Nitric Oxide Synthase (NOS) Activity Assay Kit (Colorimetric)

产品说明

一般描述

Nitric oxide synthases (NOS) are a family of enzymes that catalyze the production of nitric oxide (NO) from L-arginine. Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune response and apoptosis. In the presence of NADPH, FAD, FMN, (6R)-5,6,7,8-tetrahydrobiopterin, calmodulin and heme, NOS catalyzes a five-electron oxidation of the guanidino nitrogen of L-arginine with molecular oxygen to generate NO and L-citrulline. There are three isoforms of NOS: endothelial (eNOS), neuronal (nNOS), and inducible (iNOS). nNOS accounts for the production of NO in the central nervous system, where NO participates in cell communication and information storage. eNOS produces NO in blood vessels and is involved in regulation of vascular function. In contrast to other isoforms, iNOS is expressed de novo under oxidative stress conditions and produces large amounts of NO as part of the body′s defense mechanism.

特点和优势

Nitric Oxide Synthase (NOS) Activity Assay Kit:

• Compatible with high-throughput handling systems.
• Sensitive, detecting as low as 5 μU of NOS activity.

适用性

The kit is suitable for the detection of NOS activity in purified recombinant protein and tissue or cell extracts.

原理

In this assay, nitric oxide generated by NOS undergoes a series of reactions and reacts with Griess Reagent 1 and 2 to generate a colored product with a strong absorbance at 540 nm

产品性质

安全信息

象形图
警示用语：	Danger
危险声明	H272 - H290 - H301 - H315 - H319 - H341 - H351 - H361d - H373 - H400
预防措施声明	P202 - P210 - P273 - P301 + P310 - P302 + P352 - P305 + P351 + P338
危险分类	Acute Tox. 3 Oral - Aquatic Acute 1 - Carc. 2 - Eye Irrit. 2 - Met. Corr. 1 - Muta. 2 - Ox. Liq. 3 - Repr. 2 - Skin Irrit. 2 - STOT RE 2
靶器官	Nervous system
储存分类代码	5.1B - Oxidizing hazardous materials
WGK	WGK 3