产品说明
一般描述
The Target ID Library enables bench-top transcriptome-wide human miRNA and ncRNA gene target identification. It has an innovative dual selection system, which makes rapid whole transcriptome miRNA and ncRNA gene target screens accessible to any researcher with minimal reagent, time, or capital equipment expense.
The Target ID Library is a transcriptome wide cDNA pool produced using a proprietary process. Each library is tested for gene representation to ensure robust library coverage. The library is provided in ready-to-use DNA format, with enough material for multiple genome-wide screens.
The Target ID Library is a pool of plasmids, each with a human cDNA inserted into the 3′-UTR region of a thymidine kinase-zeocin fusion protein (Tkzeo). Dual-selection allows the user to transfect cells and screen the entire library at once, selecting first for stable transformants and secondly, after introducing a miRNA or ncRNA of interest, for target cDNAs. Selected cDNAs are identified by sequencing
Download deep sequencing data of target ID library.
组分
Library: 100μl (approx 1μg/μl), frozen liquid, store at -10℃ to -25℃.
Primer 1: 200μl (25μM), frozen liquid, store at -10℃ to -25℃
Primer 2: 200μl (25μM), frozen liquid, store at -10℃ to -25℃
法律信息
MISSION is a registered trademark of sigma-aldrich Biotechnology LP and sigma-aldrich Co.
MISSION is a registered trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.51 |
产品性质
| 质量水平 | 200 |
| 包装 | pkg of 100 μL (library) pkg of 200 μL (primer 1) pkg of 200 μL (primer 2) |
| 浓度 | 1 ng/μL (library) 25 μM (primer 1) 25 μM (primer 2) |
| 运输 | wet ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
