产品说明
一般描述
MCF10A CELLS PTEN (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.
This cell line corresponds to ATCC Catalog No. CRL-10317.
应用
Cell freezing medium-DMSO 1X, Cat. No. C6164
Media renewal changes two to three times per week.
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.
Rapidly thaw vial by gentle agitation in 37℃ water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°
特点和优势
Zinc finger nuclease (ZFN) knockout on chromosome 10q23.31
These MCF10A cells are adherent with a doubling time of approx. 20 hours
质量
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
基本信息
| eCl@ss | 32190102 |
| NACRES | NA.81 |
产品性质
| 质量水平 | 200 |
| 生物来源 | human female mammary glands (Source Disease: Fibrocystic Disease) |
| OMIM登记号 | 601728 |
| 储存温度 | −196℃ |
| Gene Information | human ... PTEN(5728) |
组份列表
组份可单独销售
| 货号 | 组份 |
| MCF10A CONTROL CELLS 1 vial | |
| MCF10A CELLS PTEN (-/-) 1 vial |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
