产品说明
一般描述
The Extract-N-Amp™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.
应用
Extract-N-Amp™血液PCR试剂盒已用于从基因组和干血斑中提取DNA。它已用于聚合酶链反应(PCR)。
特点和优势
- Efficient 8 minute prep allows greater speed and throughput
- No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
- Simple, 3 step procedure with no special equipment required
- Hot start antibody included for highly specific PCR amplification of genomic DNA
- Compatible with multiple format (single tube or 96-well plate)
- Can be used with whole blood or blood cards
- Extract stable at 4 ℃ for at least 6 months
原理
只需加入Extraction Solution并在室温下孵育5分钟，即可从10 μl全血中提取出基因组DNA。向提取物中加入Neutralization Solution，以便在PCR反应前中和抑制物。随后选取一部分DNA提取物，加入专门配制的PCR ReadyMix。
其他说明
更多信息，请见www.sigma-aldrich.com/extract-n-amp。
法律信息
凡购买本产品，即表示授权用户使用产品说明书所指定的专利，但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示，或通过禁止反言形式转让任何其他专利权(如，5′ Nuclease Process专利权)。有关购买许可的更多信息，可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利，许可抗体用于体外研究用途。
Extract-N-Amp is a trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC
基本信息
| NACRES | NA.55 |
产品性质
| 用途 | sufficient for 100 amplifications sufficient for 100 extractions sufficient for 100 reactions |
| 质量水平 | 200 |
| 特点 | dNTPs included hotstart |
| technique(s) | PCR: suitable |
| 颜色 | colorless |
| web metadata keyword.default | XNAB2R XNAB2RE |
| 运输 | wet ice |
| 储存温度 | −20℃ |
组份列表
组份可单独销售
| 货号 | 组份 |
| L3289 | Lysis Solution for Blood |
| N9784 | Neutralization Solution for Blood |
| P8115 | Extract-N-Amp™ PCR ReadyMix™ for Blood |
安全信息
| 象形图 |  |
| 警示用语： | Danger |
| 危险声明 | H290 - H314 |
| 预防措施声明 | P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363 |
| 危险分类 | Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B |
| 储存分类代码 | 8A - Combustible, corrosive hazardous materials |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
