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Extract-N-AmpTM Blood PCR Kit

Extract-N-AmpTM血液PCR试剂盒

品牌
Sigma-Aldrich
货号
XNAB2
规格纯度
sufficient for 100 extractions, sufficient for 100 amplifications
参考价格
3847.91 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

The Extract-N-Amp Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.

应用

Extract-N-Amp血液PCR试剂盒已用于从基因组和干血斑中提取DNA。它已用于聚合酶链反应(PCR)。

特点和优势

  • Efficient 8 minute prep allows greater speed and throughput
  • No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
  • Simple, 3 step procedure with no special equipment required
  • Hot start antibody included for highly specific PCR amplification of genomic DNA
  • Compatible with multiple format (single tube or 96-well plate)
  • Can be used with whole blood or blood cards
  • Extract stable at 4 ℃ for at least 6 months

原理

只需加入Extraction Solution并在室温下孵育5分钟,即可从10 μl全血中提取出基因组DNA。向提取物中加入Neutralization Solution,以便在PCR反应前中和抑制物。随后选取一部分DNA提取物,加入专门配制的PCR ReadyMix。

其他说明

更多信息,请见www.sigma-aldrich.com/extract-n-amp。

法律信息

凡购买本产品,即表示授权用户使用产品说明书所指定的专利,但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示,或通过禁止反言形式转让任何其他专利权(如,5′ Nuclease Process专利权)。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利,许可抗体用于体外研究用途。
Extract-N-Amp is a trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC

基本信息

NACRESNA.55

产品性质

用途 sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions
质量水平200
特点dNTPs included
hotstart
technique(s)PCR: suitable
颜色 colorless
web metadata keyword.defaultXNAB2R
XNAB2RE
运输wet ice
储存温度−20℃

组份列表

组份可单独销售

货号组份
L3289Lysis Solution for Blood
N9784Neutralization Solution for Blood
P8115Extract-N-Amp PCR ReadyMix for Blood

安全信息

象形图GHS05
警示用语:Danger
危险声明H290 - H314
预防措施声明P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
危险分类Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
储存分类代码8A - Combustible, corrosive hazardous materials
WGKWGK 3
闪点(F)Not applicable
闪点(C)Not applicable

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