产品说明
特异性
Green Fluorescence Detection Reagents
Use appropriate laser for λex 495 nm excitation.
Use appropriate filter for λem 527 nm emission.
Application Note
Primary antibodies are needed. Test your primary antibodies (IgG-class, mono- or polyclonal) in a standard immunofluorescence (IF), immunohistochemistry (IHC), or immunocytochemistry (ICC) assay to determine the optimal fixation, blocking, and titer conditions. Flow validated antibodies are recommended.
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应用
Based on proximity ligation assay (PLA), the Duolink® PLA technology allows for endogenous detection of protein interactions, post-translational modifications, and protein expression levels at the single molecule level in fixed cells.
Duolink® flowPLA Starter Kits will enable sensitive detection of proteins, protein-protein interactions, and protein modifications within cell populations by flow cytometry. To perform a Duolink® flowPLA experiment, you will need fixed, suspended cells, two primary antibodies that specifically recognize your proteins of interest and the Duolink® flowPLA starter Kit. The flowPLA Starter Kits are available with 3 different fluorophores: Violet, Green, and FarRed. The flowPLA starter Kits contain all the necessary reagents reagents for 100 Duolink® PLA reactions, including a pair of PLA probes (Anti-Rabbit PLUS and Anti-Mouse MINUS) and all the reagents to perform the amplification and detection of bound PLA probes by flow cytometry. Analysis is carried out using standard flow cytometry assay equipment. User must provide a fixed cell suspension, (rabbit and mouse-raised) primary antibodies, and corresponding PLA Probes.
Follow the Duolink® PLA Flow Cytometry Protocol to use this product.
Visit our Duolink® PLA Flow Cytometry page on how to run a Duolink® flow experiment, applications, troubleshooting, and more.
特点和优势
- Analyze protein protein interactions with flow cytometry readout
- Analyze cell populations with Proximity Ligation Assay
- Increased sensitivity due to rolling circle amplification for low abundant targets
- No overexpression or genetic manipulation required
- Relative quantification possible
- Works with any flow cytometer instrumentation
- Easy to follow flexible protocol
- Publication-ready results
组分
Duolink® flowPLA Detection Kit - Green DUO94002-40TST
Duolink® In Situ PLA™ Probe Anti-Mouse MINUS DUO92004-40TST
Duolink® In Situ PLA Probe Anti-Rabbit PLUS DUO92002-40TST
Duolink® In Situ Wash Buffer, Brightfield (DUO82047-4L)
See datasheet for more information.
法律信息
Duolink is a registered trademark of sigma-aldrich Co. LLC
PLA is a registered trademark of sigma-aldrich Co. LLC
Duolink is a registered trademark of sigma-aldrich Co. LLC
P3000 is a trademark of Life Technologies Corporation
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产品性质
| 质量水平 | 200 |
| technique(s) | flow cytometry: suitable immunofluorescence: suitable proximity ligation assay: suitable |
| 荧光 | λex 495 nm; λem 527 nm |
| 适用性 | suitable for fluorescence |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
