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CRISPR GUS GAPDH Reporter Control for Monocots

品牌
Sigma-Aldrich
货号
CRISPRPL01
包装型号
规格纯度
参考价格
3683.8 *本价格含增值税费
促销
数量
-+
产品介绍:

产品说明

一般描述

All-in-one, ready-to-use Cas9 and guide RNA (gRNA) expression plasmids with GUS Reporter
CRISPR Plant Cas9 products are intended for Agrobacterium-mediated plant transformation. The products are based on the type IIA CRISPR-Cas9 derived from Streptococcus pyogenes. The native Cas9 coding sequence is codon optimized for expression in monocots and dicots, respectively. The monocot Cas9 constructs contain a monocot U6 promoter for sgRNA expression, and the dicot Cas9 constructs contain a dicot U6 promoter.
Arabidopsis seedlings were germinated in 6 well tissue culture plates. The seedlings were infected with Agrobacterium which had the CRISPR plasmids with a GUS reporter. After 3-4 days of transfection the GUS expression was detected. b-glucuronidase (GUS) is an enzyme that hydrolyzes colorless glucuronides to yield colored product

应用

  • To verify successful integration of T-DNA in plant genome
  • GUS receptor wheat gGAPDH control for monocots for Agrobacterium mediated transformation

特点和优势

  • Low cost, genome editing option compared to other methods.
  • Easy to use
  • Online ordering
  • Ready to ship in 2 days

组分

1管含50μl的20ng/μl质粒DNA
在不使用时,将试管盖扣紧。
利用无菌实验技术,避免DNAase污染。

原理

CRISPR/Cas systems are employed by bacteria and archaea as a defense against invading viruses and plasmids. Recently, the type II CRISPR/Cas system from the bacterium Streptococcus pyogenes has been engineered to function in eukaryotic systems using two molecular components: a single Cas9 protein and a non-coding guide RNA (gRNA). The Cas9 endonuclease can be programmed with a single gRNA, directing a DNA double-strand break (DSB) at a desired genomic location. Similar to DSBs induced by zinc finger nucleases (ZFNs), the cell then activates endogenous DNA repair processes, either non-homologous end joining (NHEJ) or homology-directed repair (HDR), to heal the targeted DSB.

其他说明

如需订购的定制的CRISPR植物产品,请访问:CUSTOM ORDERING FORM

法律信息

CRISPR Use License Agreement

产品性质

质量水平200
重组expressed in E. coli
包装vial of 50 μL
浓度20 ng/μL in TE buffer; DNA (1μg of plasmid DNA)
application(s)CRISPR
selectionkanamycin
运输dry ice
储存温度−20℃

安全信息

储存分类代码12 - Non Combustible Liquids
WGKWGK 1
闪点(F)Not applicable
闪点(C)Not applicable

Sigma-Aldrich

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