产品说明
一般描述
OCT4 is the key transcription factor (TF) that maintains pluripotency of embryonic stem cells and plays a critical role in reinstating cellular pluripotency. The expression of OCT4 is stringently silenced in differentiated cells. Activation of silenced OCT4 gene has become a hallmark event during epigenetic reprogramming into iPSCs. The positive control OCT4 gRNA design has been tested with the dCas9-p300 plasmid in HEK293 cells and shown to increase OCT4 mRNA transcript levels over three-fold compared to a negative control.
应用
Functional Genomics/Target Validation
- Epigenetic Modification
- Transcriptional Activation
特点和优势
Serves as an experimental control for the CRISPR dCas9-p300 activation system. A positive experiment will result in an increased level of mRNA via qPCR or TaqMan PCR assay.
组分
1 vial containing 1ug of U6-gRNA plasmid expressing a guide sequence to human OCT4 (Octamer-binding transcription factor-4 or POU5F1).
联系
To order U6-gRNA plasmids, please visit sigma.com/crisprs
Click here for more information on dcas9-p300 Gene Activator Expression Plasmid.
其他说明
Must be used with dCas9-p300 or other dCas9 activator/repressor plasmids.
法律信息
CRISPR Use License Agreement
Lentiviral and WPRE License Agreements
产品性质
| 质量水平 | 200 |
| 包装 | vial of 50 μL |
| 浓度 | 20 ng/μL in TE buffer; DNA (1μg of plasmid DNA) |
| application(s) | CRISPR |
| 运输 | dry ice |
| 储存温度 | −20℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
